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enzyme can bind but only one divalent cation in the Lively internet site. For the binuclear steel center, the activation in the amide bond and the activation on the solvent h2o may be distributed separately between the two steel ions. Nonetheless, in the mononuclear metal Middle, both equally capabilities need to be borne by a single divalent cation. These final results spotlight the significant variety for your evolution of purpose in the amidohydrolase superfamily.
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cat vs. pH profile, only one ionization is noticed for both Zn- or Cd-NagA. This ionization should symbolize the protonation of the steel-certain hydroxide that is certainly used for substrate hydrolysis or, alternatively, the overall base that may be utilized to deprotonate the steel-certain water molecule previous to substrate hydrolysis. The p
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